Whole Slide Imaging: Cytoarchitecture and Connections - Two Years Later
Harvey J. Karten
Friday, October 26, 2012
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Chapters
Introduction
Hubble Image
Visual Cortex
WSI
BrainMaps & WSI
The Importance of Mesoscopic Imaging (0.1X -100X)
A Hubble for the Brain
What is Whole Slide Imaging?
Origins of WSI
Imaging of Brains Over the Past 140 Years
Some Constraints
Limits of Optical Resolution
Limits of “Capture Device ” Resolution
Limits of Display Resolution
Lessons from the Hubble
Brain Architecture: Connectomics, Molecular Architecture, IHC, ISH
Examples of Brodmann's Areas
Formalization of Cytoarchitecture
Brodmann Areas
What Now Happens
Recent Advances
Fluorescence & DAPI Stain
Pseudo Rabis Virus
Dark Field Imaging
Dark Field w/ Refraction/ Diffraction/ Scatter
Dark Field Methods
Dark Field Internal Reflection Imaging (DIRI)
Bright Field Staining and Dark Filed Condenser
Other Applications
Extracting Data from WSI
Neurolucida Software
GFP Coupled Series
Auto-Contour
Auto-Count
Applications of WSI and Data Extraction/Analysis
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