Getting the Most from Your iPS Cell Cultures
John Pulliam, PhD
Thursday, June 20, 2013
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Chapters
Introduction
Who We Are
What Are iPSCs?
The Promise of iPSCs
Reprogramming Methods
The iPSC Lines that ATCC Offers
ATCC Collection
ATCC iPSCs Are Monitored for Markers of Pluripotency
ATCC iPSCs Are Tested for Their Capacity to Differentiate into the Three Germ Layers
ATCC Reprogramming Methods Do not Affect Differentiation Potential
Overview of the ATCC Feeder-free System
Using the ATCC Feeder-free System to Successfully Sulture iPSCs
1. Preparation of CellMatrix (ACS-3035) – Coated Plates
1. Thawing and Initiating ATCC iPSC Iultures I
2. Thawing and Initiating ATCC iPSC Iultures II
2. Thawing and Initiating ATCC iPSC Cultures III
Note: Cell Morphology May Change in the Presence of ROCK Inhibitor
2. Thawing and Initiating ATCC iPSC Cultures
3. Passage or Freeze ATCC iPSC Cultures I
3. Passage or Freeze ATCC iPSC Cultures II
3. Passage or Freeze ATCC iPSC Cultures III
CoolCell®LX Alcohol-Free Cell Cryopreservation Container (ACS-6000)
Resources for iPSC Culture
Thank You
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