High Resolution GC-MS Application: Clinical Metabolomics Case study: Chronic Kidney Disease
Dr. Vladimir Tolstikov
Wednesday, July 17, 2013
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Chapters
Introduction
Metabolomics Workflow
Workflow
Lilly Metabolomics Platform
Lilly Metabolomics Platform: Data Analysis and Visualization
Daily QC & RI
Human urine GC/MS profiling
Mouse CSF GC/MS profiling
Metabolomics study requirements for GC/MS instruments
High Resolution, High Mass Accuracy: YES or NO ID
High Resolution, High Mass Accuracy: YES or NO ID (2)
High Resolution, High Mass Accuracy: YES or NO ID (3)
GC-TOF-MS data processing
Conclusions
High Resolution, High Mass Accuracy: YES or NO ID (3)
Case study: Chronic Kidney Disease
Male versus female
Uremic toxins accumulation in blood plasma
Diabetics versus non-diabetics
Integration omics data characterizing CKD
Experimental data form literature
Kidney biopsies CKD levels: Control,II-IV,ESRD
Plasma CKD
Kidney biopsies CKD levels: Control,II-IV,ESRD
Plasma CKD
Plasma CKD (2)
Plasma CKD (3)
Plasma CKD (4)
Plasma CKD (3 Larger)
Conclusions
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