High Throughput RT-qPCR for Identifying Activators of Latent HIV-1 in Primary Human CD4+ T Cells
Helen Yu, Ph.D.
Friday, January 24, 2014
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Chapters
Introduction
HIV Life Cycle
Residual Viremia Persist in Patients on HAART
Multiple Mechanisms to Activate Latent HIV
HTS Screening Cascade
High Throughput In Vitro HIV Latency Assay Reporter Gene and HIV vRNA RT-qPCR
One-Step HIV Gag RT-qPCR
Effect of Culture Medium and Lysis Method on RT-qPCR Sensitivity
Optimizing Lysate and Reaction Volumes
Correlation of vRNA activation with Reporter Gene activity
HTS hit confirmation by vRNA RT-qPCR
Summary & Conclusions
Acknowledgments
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