Enabling 3D Cell Culture Methods and Applications
Brad Larson
Tuesday, January 13, 2015
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Chapters
Introduction
Outline
Introduction of Cytation 5
Cytation 5- Filter & Monochromator
Cytation 5- Built-in Inverted Fluorescence Microscope
Cytation 5- Fully Automated Microscopy
Cytation 5- Labware: Multi-vessel Compatibility
Cytation 5- Imaging & Microscopy: Time Lapse
Cytation 5- Imaging & Microscopy: Montage and Stitching
Cytation 5- Z-Stacking, Z-Projection
MultiFlo FX- Automated Multi-Mode Reagent Dispensing & Microplate Washing
Signal Transduction in 3D Cell Culture
Real Architecture for 3D Tissue (RAFT) Procedure
Automated elF4E Assay Procedure Validation
2D/3D Cell Culture Inhibitor Comparison: p-elF4E Assay
2D/3D Cell Culture Inhibitor Comparison: Total elF4E Assay
Monitoring Spheroid Formation by Bright Field Imaging
Automated Cell Dispensing and Cell Aggregation Tracking
Image-based Spheroid Formation Tracking: ULA Plates
Toxicity in 3D Cell Culture
2D/3D Uninhibited Well Long-term Incubation Analysis
Day 10 Diclofenac Treatment Cytotoxicity Images
3D Cytotoxicity CellTox Green Diclofenac Treatement Results
Day 3 Diclofenac Treatment Oxidative Stress Images
Cytotoxicity Mechanism Assessment: Oxidative Stress
Day 7 Diclofenac Treatment MTP Assay Images
Cytotoxicity Mechanism Assessment: Apoptosis
Hypoxia Monitoring in 3D Cell Culture
Cyto-ID Hypoxia Reagent Monochromator-Based Analysis
Cyto-ID Hypoxia Reagent Image Analysis
Cyto-ID Hypoxia Reagent Cellular Analysis
Spheroid-Based 3D Tumor Invasion
MDA-MB-231/Fibroblast Tumoroid Invasion
Invadopodia Analysis
Cellular Analysis of Invadopodia
Total Invasion Analysis
Quantification of Total Invasion
Z-Projection of Invading Structure
Inhibition of Tumor Invasion
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