Quantification of human CSF tau protein without immunocapture using Triple Quadrupole mass spectrometer
Jerome Vialaret
Friday, August 7, 2015
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Chapters
Introduction
Biochemistry Laboratory – Platefome de protéomique Clinique (PPC)
Jerome Vialaret - Engineer
Quantification of tau protein in human CSF using μLC-SRM
Background: Tau protein in Alzheimer Disease (AD)
ELISA is the reference method, BUT the information obtained is limited
Bibliography
Development of an original tau extraction method
Aim of this work
Workflow
MRM Optimisation (std)
MRM Optimisation (matrix)
LC Optimisation > CSF-Sample Preparation > Analytical performance
Analytical Performance
Example of GAAPPGQK 156-163
Analytical Validation - Summary
Analytical Conclusion
7 peptides followed including ELISA epitope
Conclusions
Perspectives
Thank you
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