Large scale analysis of primary and volatile metabolism by GC-TOF MS
Professor Oliver Fiehn
Monday, July 13, 2015
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Chapters
Introduction
How do we measure the metabolome?
Why is GC-MS the gold standard for metabolomics?
GC uses retention index instead of “alignments”
GC injection is the critical process: use cold injection
Which MS libraries should we use for GC-MS based metabolomics?
Leco-Fiehn GC/MS library
Largest problem: N-Si is not as stable as O-Si
Use Quality Controls
How to avoid ‘matrix’? Exchange liners using Gerstel ALEX
GC-TOF MS enables Automatic Mass Spectral Deconvolution: Leco’s ChromaTOF
Technology showcase volatile profiling by Gerstel SPME with Leco GC-TOF MS of 3 food plates
Volatile profiling by solid-phase microextraction
Automation of SPME-GCTOF MS volatile profiling
Manual investigation SPME-GCTOF MS volatile profiling: peak MS deconvolution
Manual investigation SPME-GCTOF MS volatile profiling: food plates
Leco ChromaTOF software automatic output
Leco ChromaTOF software automatic output + WCMC statistics
Automated liner exchange – cold injection GCTOF MS primary metabolism profiling
Primary metabolites – ChromaTOF output BinBase data filtering, compound ID
ChromaTOF + BinBase automatic output
Automated liner exchange – cold injection GCTOF MS primary metabolism profiling
Integration of results from different platforms: MetaMapR
Integration of results from different platforms: MetaMapR – CA plate vs USA plate
Integration of results from different platforms: MetaMapR – Davis plate vs USA plate
GC-TOF MS for sensitive and robust quantification of keto- and amino acids
Conclusions
Go to the booth!
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