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Single Cell Analysis: Sample to Insight - Overview, Challenges and Solutions, and A Case Study

Dr. Anika Joecker
Monday, November 16, 2015
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Chapters

  • Introduction
  • Why single cell analysis?
  • QIAGENs sample-to-insight offering for single cell analysis
  • Whole genome amplification (WGA)
  • GeneRead DNAseq Targeted Panel V2
  • Biomedical Genomics Workbench – Complex Tasks, Simply Done
  • Specific functionality available in Biomedical Genomics Workbench
  • Variant calling with Biomedical Genomics Workbench
  • QIAGEN Bioinformatics Products Streamline Integration
  • Ingenuity Variant Analysis
  • Introduction
  • Case story 1 : REPLI-g vs. MALBAC pt. 1
  • Case story 1: REPLI-g vs. MALBAC pt. 2
  • REPLI-g vs. MALBAC – visualized mapping result
  • MALBAC has a very high coverage around 4.31M
  • REPLI-g Single Cell WGA
  • Case story 2 : Sample-to-insight
  • Case study 2: Complete sample-to-insight workflow
  • Allele Frequency for Heterozygous Sites (LoVo)
  • Data interpretation
  • Validation of MiSeq results using Pyrosequencing
  • Summary
  • Thank You
 

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