Miniaturization and Automation of Single-Cell RNAseq Library Preparation
Louise Laurent, MD. PhD
Thursday, February 4, 2016
Tweet
Transcript
Embed
Email
Bookmark
<<
<
>
>>
Tweet
Facebook
Email
Chapters
Introduction
The Potential of Single-Cell Transcriptome Sequencing
The Challenges of Single-Cell Transcriptome Sequencing
Questions to answer
Overall Approach to establishing reproducibility and validity
Single-cell cDNA synthesis
mosquito HTS and mosquito HV
Single-cell RNAseq library construction
Technical Variability
Ability to Recognize Biological Variability
High Degree of Overlap in Detected transcripts in technical replicates
High Degree of Overlap in Detected transcripts in different reaction volumes
Reproducible identification of transcripts specifically expressed by individual cells
End-to-end automation of workflow
Results from Single-Cell RNAseq of A1:F20 beta cell differentiation timecourse
NMF Visualization
Dendrogram
No transcript for this webinar
More Information
Request info