Transcriptome analysis of Kupffer cells after uptake of pancreatic cancer exosomes reveal pathways and biological processes involved in metastatic progression
Jean-Noel Billaud, PhD
Friday, May 13, 2016
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Chapters
Introduction
Legal disclaimer
Keywords
Transcriptome of Kupffer cells after PDAC exosomes uptake
OUTLINE of the presentation
Pancreatic Ductal Adenocarcinoma (PDAC)
Many clinical trials
Most commonly affected genes and pathways in PDAC
QIAGEN Sample to Insight
Biogenesis, secretion, and intercellular communication of exosomes
Exosomes in cancer progression
Functional relevance of exosomes in cancer
Pancreatic cancer exosomes initiate pre-metastatic niche formation in the liver
Tumor exosome integrins determine organotropic metastasis
Description of the study: SRP058375
Simple to use RNA-Seq solution (FASTQ to Insight)
Introduction to Biomedical Genomics Workbench (BxWB), data analysis
BxWB to IPA: data analysis to interpretation, FASTQ to Tracks
BxWB to IPA: data analysis to interpretation, Tracks to STATS
Differential expression for RNA-Seq Analysis
Analyze Expression Data and Upload Comparisons to IPA
Differential expression for RNA-Seq analysis
How to study 100’s or 1000’s of genes and proteins at the same time?
Ingenuity was founded ~15 years ago to help solve this problem
Significant Recent Milestones for IPA
Over 5 million findings to date
Introduction to Ingenuity Pathway Analysis (IPA), Interpretation
IPA Core Analysis
Canonical Pathways and their Pathway Activity Analysis pt.1
Canonical Pathways and their Pathway Activity Analysis pt.2
Comparison of Canonical Pathways across the conditions w/wo ITGB5
IL-6 signaling report
IL6 signaling is involved in differentiation of leukocytes
Upstream Regulator Analysis of Kupffer Cells after uptake of BxPC-3
IL6 is involved in tumor progression induced by BxPC-3 exosomes
Comparison of Upstream Analysis across conditions w/wo ITGB5
TRIM24 influences the immune responses after uptake of BXPC-3
MEOX2 is predicted to be inhibited after uptake of BxPC-3
Comparison of Diseases & Functions across conditions w/wo ITGB5
ITGB1 isoforms: player in fibrosis after uptake of BxPC-3
Detection of Isoforms that could increase HCC after BxPC-3 uptake
Pyruvate Kinase Isoforms and their relative expression across conditions
Causal Network linking IL6R and Metastasis
Hypothesis to inhibit induced metastasis after uptake of BxPC-3 by KC
Causal Network linking IL11RA and Metastasis
Hypothesis: inhibition of IL11RA
Transcriptome in induced metastasis after PDAC exosomes uptake
Liquid biopsy solutions at QIAGEN – Sample to Insight
Characterization of RNA from Exosomes
Isolation of exosomes and exoRNAs
Acknowledgements
QIAGEN Bioinformatics at AACR
Welcome to 3 part webinar series on Digital RNA sequencing
QIAGEN Bioinformatics – RNA-seq Summit
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