Discovering volatile biomarkers using GC×GC-TOFMS
Detecting and phenotyping infections directly from lung samples
Dr. Heather Bean
Wednesday, June 29, 2016
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Chapters
Introduction
Pathophysiology of Cystic Fibrosis
Pseudomonas aeruginosa
P. aeruginosa mutates in the CF lung
Prevention is the best treatment for chronic infection
Diagnosing Lower Respiratory Infections
Hypothesis 1: Pseudomonas core VOCs
Hypothesis 2: Phenotype-Specific VOCs
Progression & complexity
Hypothesis 1: Pseudomonas core VOCs Pt2.
Diagram 1.
Diagram 2.
Diagram 3.
25 clinical P. aeruginosa isolates
Sampling and Analysis
Diagram 4.
Chemical Class
Diagram 5.
Cystic fibrosis sputum with P. aeruginosa
Diagram 6.
Pan metabolome accumulation curve
Core metabolome rarefaction curve
Complete P. aeruginosa volatile metabolome
Hypothesis 2: Phenotype-Specific VOCs Pt2.
P. aeruginosa mutates in the CF lung
Detecting P. aeruginosa mucoidy
Complexity, Clinical Utility
Hypothesis & Approach
Stress
Phenotypic switching, early infection isolates
mucA mutation hotspot, chronic infection isolates
VOCs of mucoid and non-mucoid P. aeruginosa
Some VOCs respond oppositely to phenotype
Diagram 7.
Combination of VOCs = Phenotype & Genotype
Diagram 8.
Acknowledgements
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