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Promega DNA QC Assay: Strategies for Predicting Downstream NGS Success with FFPE Samples

Curtis Knox
Monday, November 28, 2016
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Chapters

  • Introduction
  • NGS Workflow-Where Can QC Make a Difference?
  • Comparative FFPE Experiments for NGS
  • qPCR Quantitation of FFPE Samples
  • Does Quantification Equal Library Yield?
  • Does Quantification Predict Coverage Uniformity?
  • Does Library Yield Equal Coverage Uniformity?
  • DNA QC Assay-System Information (In Development)
  • DNA QC Assay for FFPE/ccfDNA
  • Does Degradation Predict Library Yield?
  • Degradation Ratio-The Best Predictor
  • Why is Fragment Size So Important?
  • Accel-Amplicon 56G with FFPE DNA Extracted After Different Fixation Times
  • Accel-Amplicon 56G with Horizon's FFPE Quantitative Multiplex Reference Standard
  • Summary
 

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