Automated High Throughput Flow Cytometry: Live Cell Cytometry and Phenotypic Screening
Dave Gebhard
Wednesday, March 1, 2017
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Chapters
Introduction
Mission statement
High Content Screening and Cytometry Facility Groton, CT
Automated High Content Robotic System for High Throughput Cytometry
Evaluating Phenotypic Screens
Evaluating Phenotypic Screens– 3 Key Criteria
Pharmacologic Chaperone- Assay for Increased Expression
Assay workflow
Chaperone - dual reporter QC and Internal controls
7000 compounds Screen (2 runs) duplicate single points
Human Primary Cells Phenotypic Screening
Human Primary Cells Phenotypic Screening
Informatics Overview
Data Work Flow
The Team
Our In Vitro Pharmacology Department is Hiring!
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