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Antibody Characterisation at MRC Technology

Rachel Forfar, PhD
Wednesday, March 1, 2017
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Chapters

  • Introduction
  • FORMING PARTNERSHIPS
  • COLLABORATION
  • IntelliCyt @ MRCT
  • IntelliCyt applications @ MRCT
  • Antibody-Drug-Conjugates
  • Antibody-Drug-Conjugate targets
  • 1) Antibody binding
  • 1) Cell binding: flow cytometry sec 1.
  • 1) Cell binding: flow cytometry sec 2.
  • 1) Cell binding: flow cytometry sec 3.
  • 2) Screening hybridoma supernatants
  • ALK: Expi293 cells (overexpressed)
  • 3) Antibody internalisation
  • 3) Antibody internalisation: flow cytometry & imaging
  • Promega on-bead conjugation – pH dye (Promega)
  • 3) Internalisation: flow cytometry
  • Acid wash method, strips surface binding
  • Promega on-bead conjugation – screening ALK hyb sups
  • 3) Flow internalisation of A488 conjugates
  • IntelliCyt applications @ MRCT
  • Acknowledgements

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