Replacing LECO Pegasus HT with Pegasus BT GC/TOF-MS Instruments
Jan Hazebroek, PhD
Monday, July 16, 2018
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Chapters
Introduction
Why metabolomics?
Metabolomics highly automated
We apply established methods
Harvesting samples
We follow established analytical protocols
Metabolomics core infrastructure
Data flow
Baseline correction and alignment by Genedata software
Internal standard evaluation using Genedata software
Metabolomics sample capacity
Leco Pegasus BT
Advantage #1: New
Advantage #2: Sensitive
Advantage #3: Dynamic range
Advantage #4: Mass resolution
L-Pal3 autosampler: Just-in-time on-line trimethylsilylation
Integration of ChromaTof BT software
Genedata Refiner MS (data processing)
Example applications
Plant disease greenhouse experiment
Metabolite with high fold change and low P value is a treatment biomarker
Genedata Analyst
Classifying resistance by discriminant analysis (inoculated)
Classifying resistance by discriminant analysis (non-inoculated)
Plant herbivory greenhouse experiment
Strong treatment effect
Resistance to infestation: A phenolic acid increases
Susceptible to infestation: Two biochemically related metabolites increase
Drought stress field experiment
PCA classifies treatment and development stage
GC/MS predicts drought tolerance with drought stress #1
Acknowledgements
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