SeqCap Epi: Enabling Lower Inputs and New Applications for Targeted Methyl-Seq Analysis
Nancy Nabilsi, PhD
Tuesday, June 12, 2018
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Chapters
Introduction
Agenda
Epigenetics
Bisulfite sequencing
Agenda
Combining bisulfite sequencing with target enrichment
SeqCap Epi probe design
SeqCap Epi accurately and reproducibly quantifies CG methylation
Agenda
Reducing input amount requirements
Evaluation of HyperPrep with SeqCap Epi
Reduced inputs yield equal or better sequencing metrics
What’s so special about cfDNA?
cfDNA is a better substrate than gDNA for low-input NGS library prep
Optimizing low-input library preparation for target enrichment
Optimizing low-input library preparation for target enrichment
Optimizing low-input library preparation for target enrichment
SeqCap Epi is applicable to cfDNA samples
Agenda
Epigenetics
Sequencing techniques for genome-scale mapping of 5hmC
TAB-seq
TAB-SeqCap Epi evaluation
TAB-SeqCap Epi identifies 5hmC at expected levels
TAB-SeqCap Epi identifies 5hmC at expected levels
TAB-SeqCap Epi identifies 5hmC at expected overlaps
Summary
Acknowledgements
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