Robust library preparation for improved ultra-low input and flexible single-cell RNA-sequencing
Nancy Nabilsi, PhD
Thursday, December 6, 2018
Tweet
Transcript
Embed
Email
Bookmark
<<
<
>
>>
Tweet
Facebook
Email
Chapters
Introduction
Agenda
KAPA RNA HyperPrep
Benchmarking KAPA RNA HyperPrep performance with lower inputs
Higher library yields with KAPA RNA HyperPrep
Efficient removal of rRNA across inputs using KAPA RNA HyperPrep
Retain more unique molecules using KAPA RNA HyperPrep
Identify more transcripts using KAPA RNA HyperPrep
Excellent reproducibility down to 1 ng with KAPA RNA Hyper
Excellent reproducibility down to 5 ng with KAPA RNA Hyper
Excellent agreement across input quantities
Summary
Ultra-low input/single-cell RNA-seq
Alleviate the bottleneck of cDNA normalization
High-quality data was obtained across all inputs
HyperPlus yields more complex libraries than Nextera™ XT
Excellent agreement across 30-fold input range for HyperPlus
CellRaft Technology: Operation and Applications
CellRaft Technology
Components
Operation
CellRaft Systems
Applications of the CellRaft Technology
Gemcitabine sensitivity of CFPAC-1 Cells
CRISPR/cas9 Clonal Colony Propagation
Protocols, News and Events
Single-cell RNA-sequencing
High-complexity libraries exhibit expected genomic rates
Gene expression profiles are similar between single cells
Summary
Acknowledgements
No transcript for this webinar
More Information
Request info