Breakthrough Technologies for Quantitative and Qualitative Characterization of Biologics: Therapeutic Monoclonal Antibody Characterization Using TripleTOF ® System
Michael Jarvis
Tuesday, November 6, 2012
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Chapters
Introduction
Outline
Therapeutic Immunoglobulin Molecules
IgG1 Structural Characterization at Four Levels
Level 1 and 2 – Intact Protein MW Analysis
LCMS to Determine Intact MW of Antibodies
Excellent Sensitivity for Antibody Analysis
Accurate Molecular Weight Determination
Accurate Molecular Weight Determination I
Accurate Molecular Weight Determination II
Accurate Molecular Weight Determination III
IgG1 Structural Characterization at Four Levels
Level 3 Peptide Mapping
Peptide Mapping – TripleTOF® 5600 System
Zoom in TIC & XIC Windows
ProteinPilot™ Search Results
Peptide modification I – Deamidation
ProteinPilot™ Search Results
Peptide modification II - Oxidation
Relative Quantitation of Individual Peptides
Peptide Mapping – TripleTOF® 5600 System
N-linked Glycan Profiling
Reconstructed MS Spectrum (0 Charge State)
MS/MS Peak Interpretation
Relative Quantitation of Glycopeptides
Identification and Quantitation of Disulfide Bonds
MS/MS Data Interpretation for Sequence Confirmation
IgG Structural Characterization at Four Levels
Experimental Details
Fluorescence Dye Labeled Glycan
dentification and Quantitation of Fluorescence Dye Labeled Glycan
MS and MS/MS Data of Selected Glycan
MS/MS Data Interpretation of Fluorescence Dye Labeled Glycan I
MS/MS Data Interpretation of Fluorescence Dye Labeled Glycan II
Identification and Quantitation of Fluorescence Dye Labeled Glycan
Summary
Preview of Biopharma Beta Software
3 Processing Steps for Peptide Mapping
BiopharmaBeta software
Digesting the Protein
Biopharma Beta Software
Peptide Mapping Result
Peptide Confirmation Using Multiple Charge Series
Peptide Mapping Result
Updated Assay Information
Thank You for Your Attention
Trademarks/Licensing
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