Chromatography 101 Part 2: Introduction to Ion Exchange Chromatography
Shawn Anderson
Thursday, September 26, 2013
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Chapters
Introduction
Agenda
Ion Exchange Brief History and Theory
Ion Exchange Theory
Amino Acids: the Building Blocks of Proteins
A Typical Protein Macromolecule
Basics of Media Choices - Matrix
Basics of Matrix Choices - Ligand
Buffer pH Changes Protein Charge
pH and Buffer Selection
Common Elution Factors
Gradient Profiles
Gradient Shape
Particle Size vs. Resolution
Flowrate vs. Resolution
Capacity vs. Resolution
Secondary and Polishing of MAb
Purification Solutions from Bio-Rad
Summary
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Chromatography 101: Part 1
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