Characterization of Chemokine Receptor Antibody Function and Human Chemokine Receptor Expression
Kelly Lundsten
Monday, May 12, 2014
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Chapters
Introduction
Chemokines
Seminar Outline
Mycoplasma Negative Cell Lines
Chemokine Titration Protocol for Chemotaxis
Functional Blocking of Chemotaxis Protocol
HUT78 Chemokine Receptor Expression Screen
CXCL11 Titration Curve and α-CXCR3 Blocking
CCL17 Titration Curve and α-CCR4 Blocking
CCL1 Titration Curve and α-CCR8 Blocking
Dissociation can Affect Receptor Profile of MCF-7
CD194 Alexa Fluor 647 can be Detected on Fixed or Live Cells
CD183 BV421 Positive Staining on Cells Labeled Live but Not Fixed
Online Tools: Chemokine-Receptor Table
Chemokine Receptors Expressed on Various Cell Types in Blood
Our Newest Fortessa Configuration
Types of Fluorescent Molecules
The Brilliant Violet Family...So Far
Not all Fluors are Created Equal
Break it Down: Which Fluors for How Many ‘Colors’
Multicolor Flow Experiment: Receptor Expression Profiles in Healthy Donors
Optimization: Titrate Antibodies
Multicolor Panel Composition
Basic Phenotypic Gating Strategy
Expression of Single Chemokine Receptors on NK Cells
Fluorescence Minus One (FMO) Gating Controls on NK Panel
T-Cell Chemokine Expression
Individual Differences in CD197 Expression in T-Cell Panels
T-Cell Chemokine Receptor Coexpression
How to Gate CD192+/CD197+/CD185+
Multivariate Analysis with Cytobank SPADE
Subpopulations Co-expressing Three Chemokine Receptors
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