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Insights Begin with a Single Cell: QuantiGene® FlowRNA

Sue Reynolds
Tuesday, May 13, 2014
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Chapters

  • Introduction
  • Insights Begin With a Single Cell
  • Exploring Population Heterogeneity
  • QuantiGene® FlowRNA Assay
  • Branched DNA Technology
  • Bridge Molecular Profile with Cellular Content
  • QuantiGene® FlowRNA Assay Workflow
  • Cytokine RNA and Protein Profiling in PBMC's
  • Application Focus Areas
  • Kinetics of Transcription and Translation - Correlation of Cytokine mRNA and Protein
  • IL-23 mRNA in Polarized Human TH17 Cells
  • Co-Detection of Cytokine RNA and Protein in Bone Marrow-Derived Plasmacytoid Dendritic Cells (DCs)
  • mRNA Detection in Activated CD4+ T Cells Secreting IL-2
  • mRNA Detection in Activated CD4 T Cells Secreting IFNγ
  • Co-Infections with Hepatitis C Virus
  • Detection of Hepatitis C vRNA in Co-Infected Human Heptaocytes
  • RNA Probes Differentiate between JFH1 Strains
  • QuantiGene® FlowRNA & Translational Science
  • In Summary - Single Cell Analysis Reveals Important Biology
  • Acknowledgements
  • Thank You
 

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