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1536 qPCR: A Robust Method for Detecting Changes in Gene Transcripts

Peter Aspesi
Monday, February 23, 2015
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Chapters

  • Introduction
  • Topics to Cover
  • Developmental and Molecular Pathways (DMP)
  • Intro to HTB
  • What HTB does
  • Robotics in HTB
  • Technology - Splice Variants
  • Technology - qPCR assay
  • Workflow – 1-step or 2-step RT-qPCR
  • 2-Step
  • 1-step process
  • One step vs two step
  • Reagents
  • Instrumentation
  • LightCycler® 1536 Real-Time PCR System: System Components - Instrument
  • LightCycler® 1536 Real-Time PCR System: System Components – Multiwell Plates
  • Workflow - 1536 qPCR
  • Challenges
  • 50K Data Histogram
  • 50K Data
  • Compound Collection
  • Follow Up Data
  • Conclusion
  • Acknowledgements
 

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