Protein Trafficking and High-Speed Cytometry: A Marriage of Speed and Sensitivity for Assays not Previously Amenable to HTS
Scott Sneddon
Thursday, March 19, 2015
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Chapters
Introduction
Trafficking
Why Trafficking?
Why Not Trafficking?
The FAP-Biosensors
CFTR Assay
Ion Channel Trafficking
Positive Readout
Positive Readout versus GFP
Homogeneous Readout
Why Cytometry?
Cytometry vs Imaging
Cytometry and Speed
Descrimination (Gating)
Cytometry and Gating
The other speed, Throughput
Getting Cells into the Cytometer
The Protocol
Machine Settings
Summary
Acknowledgements
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