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SeqCap Epi: enabling lower inputs and new applications for targeted methyl-seq analysis

Nancy Nabilsi, PhD
Thursday, October 26, 2017
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Chapters

  • Introduction - Nancy Nabilsi
    • Agenda
      • Epigenetics
        • Bisulfite sequencing
          • Agenda
            • SeqCap Epi probe design
              • SeqCap Epi accurately and reproducibly quantifies CG methylation
                • Agenda
                  • Reducing input amount requirements
                    • Evaluation of HyperPrep with SeqCap Epi
                      • Reduced inputs yield equal or better sequencing metrics
                        • What's so special about cfDNA?
                          • SeqCap Epi is applicable to cfDNA samples
                            • Agenda
                              • Epigenetics
                                • Sequencing techniques for genome-scale mapping of 5hmC
                                  • TAB-seq
                                    • TAB-SeqCap Epi evaluation
                                      • TAB-SeqCap Epi identifies 5hmC at expected levels
                                        • TAB-SeqCap Epi identifies 5hmC at expected levels
                                          • TAB-SeqCap Epi identifies 5hmC at expected overlaps
                                            • Summary
                                              • Acknowledgements

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