DriverMap Transcriptome Profiling in Drug Screening Applications
Lester Kobzik, MD
Thursday, November 29, 2018
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Chapters
Introduction
Phases of Drug Screening
Screening vs. A375 Melanoma: Leads identified---now what?
Drug Screen Transcriptional Signatures
DriverMap Technology Outline
DriverMap Assay: Key Applications
Experimental Protocol
Analysis Workflow
Screening vs. A375 Melanoma: Leads identified---now what?
Transcriptome profiling: A375 24h after drug treatment
Drug Panel MOA Analysis using LINCS1000
Transcriptome profiling: A375 24h after drug treatment
Ingenuity IPA Causal Analysis of RAF Kinase Inhibition
Drug Panel Cytotoxic Effects on A549 cells at 72h
More Bioinformatic Analysis
A375 Drug Response: Down-Regulated Pathways Common & Drug-Specific
RAF Inhibitors vs A375 (Dabrafenib, Sorafenib, Vemurafenib)
Summary
Acknowledgements
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