NGS Barcoding Technologies and Targeted RNA Expression Profiling for Single-Cell Genomics
Alex Chenchik, PhD
Thursday, November 29, 2018
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Chapters
Introduction
Cellecta, Inc. and Portfolio
Complexity of Tumorigenesis Mechanisms
Challenges in Expression Profiling
Comparison of Gene Expression Technologies
What is DriverMap?
DriverMap Technology Outline
Heat Map of RNA-Seq and DriverMap Data
RNA Input and Reproducibility
Single Cell Profiling with DriverMap
DriverMap Single Cell Profiling HeatMap
DriverMap Single Cell PCA
Cell-specific Signatures
DriverMap Targeted RNA Expression Portfolio
DriverMap Assay: Key Applications
Clonal Evolution in Tumor Development
Clonal Barcode Analysis of Tumor Development
ClonTracker Barcode Libraries for Single-Cell Genomics
In vivo CRISPR Screen to Identify Regulators of Metastasis
Discovery of Metastasis Promoting Genes
Primary and Metastatic Xenograft Profiling
Single-cell Genetic Screen (Perturb/CROP-Seq)
CRISPR in vivo genetic screening in GEMM
CRISPR/barcoding enable in vivo pharmacogenomics
Relating Drugs to Genotypes to Transform Precision Cancer Therapeutics
Summary of Discussion
Acknowledgments
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