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Better Science Through Imaging

Paul Maddox
Thursday, January 3, 2019
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Chapters

  • Introduction
    • Acknowledgements
      • Light Sheet Microscopy
        • Conventional Microscopy vs Light Sheet
          • Existing light sheet microscopes use low(ish) NA detection objectives
            • Physically impossible to use light sheet with high NA objectives (using traditional objective orientation)
              • Rationale of our microscope design
                • LITE sheet microscopy
                  • Illuminating a sample on the coverslip is impossible with a perfectly flat sheet
                    • Illuminating a sample on the coverslip is possible with a tilted sheet
                      • Xenopus laevis tissue culture cells
                        • High Res Imaging In Cells
                          • High Res and Super Imaging In Cells
                            • Multi-generational nuclear lineage tracing with LITE in syncytial fungus Ashbya gossypii
                              • Lateral Interference Tilted Excitation (LITE) microscopy
                                • Conclusion

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