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Unraveling Clonal Heterogeneity and Evolutionary History of AML by Single-Cell DNA Sequencing

Koichi Takahashi, MD
Wednesday, April 24, 2019
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Chapters

  • Introduction
  • Clonal heterogeneity
  • Ecosystem
  • Clinical implications of clonal heterogeneity
  • Mutation
  • Single-cell DNA sequencing in cancer
  • Limitations from historical scDNA seq studies
  • High-throughput single-cell DNA sequencing of acute myeloid leukemia tumors with droplet microfluidics
  • scDNA seq of longitudinal AML samples
  • Baseline characteristics of the 70 AML patients
  • Number of input cells, sequenced cells, and ADO
  • Bulk VAF vs. pseudo bulk VAF
  • LOD of the scDNAseq (estimated between 0.1-0.3%)
  • Cell-level co-occurrence of multiple mutations
  • Cell-level mutual exclusivity of multiple mutations
  • Landscape of single-cell driver mutations in AML
  • Reconstructing phylogeny of driver mutations in AML
  • Divergent/Convergent evolution AML
  • Case 1-74-year-old male with t-AML with BM blast 62%
  • Case 2-58 year old man with AML
  • Summary
  • Acknowledgment

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