Lumit™ Technology for Homogeneous Bioluminescent Immunoassays
Dan Lazar, PhD
Tuesday, February 11, 2020
Tweet
Transcript
Embed
Email
Bookmark
<<
<
>
>>
Tweet
Facebook
Email
Chapters
Introduction
Presentation Outline
NanoLuc® Binary Technology (NanoBiT®)
Lumit Immunoassay Platform
Lumit Immunoassay Configurations
Lumit Cytokine Immunoassays
Cytokines
First Step to Build a Lumit Immunoassay
Rapid Screen to Identify Productive mAb Combinations
Lumit Human IFN-γ Immunoassay to Detect Released Cytokine from Cells
Lumit Human IL-1β Immunoassay
Lumit hIL-2 Immunoassay is Scalable for Screening
Lumit Cytokine Immunoassays Summary
Lumit Immunoassay Cellular System
The Crucial Role of Protein Phosphorylation in Cell Signaling
Lumit Immunoassay Cellular System (IκBα Example)
Selecting Primary Antibody Pairs for Lumit Immunoassay Cellular System
Linearity and Sensitivity of Lumit Immunoassay Cellular System
Detection of Total and Phosphorylated IκBα (and p65) upon NF-κB Pathway Activation and Inhibition
Kinetic Studies on NF-κB Pathway Activation with TNFα treatment of MCF-7 cells
Drug Modulation of NF-κB Pathway Detected with the Lumit Immunoassay Cellular System
Comparison with Other Homogeneous Cell-based Pathway Assays
High Throughput Screening (HTS) Utility of Lumit Immunoassay Cellular System (pS473-Akt)
Lumit Immunoassay Cellular System Validated with Multiple Signaling Targets
Lumit Immunoassay Cellular System Summary
Lumit FcRn Immunoassay
Role of FcRn-Antibody Interaction In Vivo
Lumit FcRn Immunoassay
Simple Protocol for Lumit FcRn Immunoassay
Lumit FcRn Immunoassay on a Panel of Therapeutic Antibodies
Lumit FcRn Immunoassay to Detect Altered Antibody-FcRn Binding
Lumit FcRn Immunoassay Specificity
High Reproducibility Within and Between 384-well Assay Plates
Lumit FcRn Immunoassay Summary
Lumit Immunoassay Platform Summary
No transcript for this webinar
More Information
Request info