Ultra-high throughput HLA genotyping for stem cell donor recruitment
Claudia Pahlke, PhD
Wednesday, April 29, 2020
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Chapters
Introduction
The Challenge: Impact of Blood Cancer
About DKMS
The founding of DKMS
More than 9.8 Mio. registered DKMS donors
Unrelated blood stem cell donations
DKMS-We delete blood cancer
DKMS LIFE SCIENCE LAB
Our ultra-high throughput lab in Dresden, Germany
Human Leukocyte Antigen (HLA)
Milestones: typed gene markers and typing method
Ultra-High Throughput workflow
DNA Isolation
DNA Quantification
Sample Reformatting and PCR Preparation
PRE PCR
PCR
Pooling of loci and barcoding PCR
Sample pooling & cleanup
qPCR quantification
Pool combination
Illumina Sequencing
POST PCR
HLA AND BEYOND
Why do we do more?
Research goes on: selection of publications
Conclusion
Thank you
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