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Cell X - An Automation Tool for iPSC and MSC Cell Fabrication

Ratnam Mantripragada
Monday, April 5, 2021
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Chapters

  • Introduction
  • Cell X, An Automation Tool
  • Cell Xˇ Applications
  • iPSC Reprograming and Performance-Based Clone Selection
  • Automated imaging and confluence assessment
  • Automated imaging to monitor and document iPSC
  • Automated weeding of fibroblasts
  • iPSC Colony with central differentiation
  • Automated iPSC Clone picking and morphometric analysis
  • Automated iPSC colony picking and expansion
  • Automated media changes, imaging and weeding
  • Overview of iPSCs to cardiomyocyte differentiation
  • iPSC differentiation to cardiomyocytes
  • Overview of iPSCs fabrication using 3D microspheres
  • Automated picking of TRA1-60 + 3D microspheres
  • Automated iPSCs expansion on 3D microspheres
  • MSC fabrication using performance-based selections
  • Single Cell Picking
  • Time Lapse Video - CTP-C heterogeneity
  • Cartilage Derived CTP-C selection
  • Final Remarks

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