Immunophenotyping of T- and B-cell Receptors
Alex Chenchik, PhD
Monday, November 21, 2022
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Chapters
Introduction
Gateway to Adaptive Immune System: TCR/BCR Repertoire Analysis
Generation of Diverse TCR and BCR Repertoire
Immunophenotyping of Adaptive T and B Cell Immune Responses
Adaptive Immune Receptor (AIR) mRNA Profiling Assay
Comprehensive AIR Repertoire Analysis in Whole Blood and PBMC
TRB Clonotype Repertoire Analysis in Blood Samples
Reproducibility in TRB top 100 Clonotype Repertoire Analysis
TRB Repertoire Analysis in PBMC-Tissue mixes
Sensitivity of TCR/BCR Profiling: RNA vs DNA samples
AIR repertoire Analysis: Detection of Ag-activated clones
AIR assay: Detection of Cancer-Activated CDR3 Clones
T/B Immunophenotyping Assay
AIR Immunophenotyping: T/B Cell Fractions vs Single-Cell Analysis
AIR & T/B Immunophenotyping Assay for T Cell Sorted Fractions
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