This page provides an in-depth overview of the InTraSeq technology, how it can empower your single cell analysis research, and product offerings.
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Single cell RNA sequencing (scRNA-seq) is commonly utilized to determine cellular responses to pharmacologic and environmental perturbations. However, protein expression levels often do not directly correlate with RNA expression, leaving a gap in the understanding of complex physiologic systems. Determining how single cell protein expression and post-translational modifications differ from single cell gene expression can offer functional insight into pathway activation that cannot be obtained from scRNA-seq alone.
Post-translational modifications (PTMs) can alter protein activity, longevity, and expression levels. Understanding the interplay between genetic expression and protein activity is crucial to determine the underlying cellular heterogeneity and functional state of complex biological systems. The InTraSeq 3' Conjugate Antibody Cocktail is formulated with 31 of our most popular antibodies that bind to both human and mouse targets, including PTMs. Scientists can quantify dozens of critical signaling proteins in a single assay with ease and without bias.
Many cells can exhibit similar levels of RNA expression, but some may differ in which proteins are ultimately expressed and subsequently modified to regulate activity after pharmacologic or environmental perturbations. Co-quantification of RNA, protein, and post-translational modifications facilitates a more thorough understanding of complex physiologic interactions that RNA expression alone cannot elucidate.