Fully Automated RNA In Situ Hybridization for Proliferation and Apoptosis-related Biomarkers in Preclinical Animal Model Tissue Panels using RNAscope® 2.5 LS Assay
Dr. Courtney Anderson
Friday, December 11, 2015
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Chapters
Introduction
Methods and Materials
Table 1. Tissues tested by RNAscope® 2.5 LS assay
RNAscope® 2.5 LS assay
Cynomolgus monkey testis FFPE tissue stained by different pretreat conditions
Table 3. Optimal pretreat condition on RNAscope® 2.5 LS
Figure 2. Detection of apoptosis-related targets in rat testis
Figure 3. Detection of cell cycle marker cyclin E1 (CCNE1) rat FFPE tissues
Figure 4. Detection of proliferation marker Ki-67 (MKI67) in multiple FFPE tissues
Figure 5. Detection of CD68 and endothelial marker PECAM1 (CD31) in multiple FFPE tissues
Original Image vs. Analyzed by Halo™
Conclusion
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